Epoch Eclipse™ Quencher

Epoch Eclipse™ quenchers are designed as broad-spectrum dark quenchers, meaning they can quench a wide range of fluorophores across the visible spectrum.

Typical quenching range:

• Approximately 500–750 nm T

This allows compatibility with many common dyes, including:

• FAM
• HEX
• TET
• Cy3 T
• AMRA
• ROX
• Texas Red
• Cy5

General fluorescence transfer concept:
Fluorophore ∗ →Quencher→Heat

The quencher absorbs emitted energy and dissipates it as heat rather than fluorescence.

Why Use Epoch Eclipse™ Quenchers

1. Low Background Signal

Because it is a dark quencher, it produces:

• little or no native fluorescence
• cleaner assay signals
• improved signal-to-noise ratio

2. Broad Dye Compatibility

One quencher can work with multiple fluorophores, simplifying probe design.

3. Improved qPCR Performance

Commonly used in:

• TaqMan probes
• hydrolysis probes
• molecular beacons

Benefits:

• higher sensitivity
• lower background
• more accurate quantification
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4. Efficient FRET Quenching

Works well in fluorescence resonance energy transfer (FRET) systems for:

• biosensors
• hybridization assays
• cleavage assays

5. Stable Oligonucleotide Modification

Can be incorporated at: 3′ end 5′ end internal positions for stable probe synthesis and detection performance.

Common Applications

• qPCR probes
• Molecular beacons
• SNP detection
• miRNA assays
• Pathogen detection
• Diagnostic probes
• Real-time PCR
• Fluorescence biosensors