Enhanced Diagnostic Tools
N-terminal Sequencing service determines the N-terminal amino acid sequence of proteins and peptides by the Edman procedure. Edman degradation is a cyclic procedure where PITC is coupled to the N-terminal amino group followed by cleavage of the N-terminal residue. The PITC coupled residue is transferred to a flask, converted to a PTH-residue and analysed by injection on an HPLC column.
Samples can be submitted in liquid form, solid form or as spots/bands from PVDF membranes.
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