Live Chat Support Software
800.227.0627

Dye and Quencher pair

lists many of the fluorescent dyes currently available with their excitation and emission wavelengths and their most optimal quencher option.

Dye-quencher compatibility

Dye Name

Ex.

Em.

BHQ Quenchers

Quenchers

Alexa 350

346

442

BHQ-0
l(MAX)=
495nm
Range=
430-520nm

QSY-35
l(MAX)=
475nm

 

DABCYL
l(MAX)=
479nm

Pacific Blue

416

451

Marina Blue

362

459

Eclipse
l(MAX)=
522nm

Acridine

362

462

Edans

336

468

Coumarin

432

472

BODIPY 493/503

493

503

Cy2

489

506

 

BODIPY FL-X

504

510

DANSYL

335

518

 

 

Alexa 488

495

519

BHQ-1
l(MAX)=
534nm
Range=
480-580nm

FAM

495

519

Oregon Green

500

520

Rhodamine Green-X

503

528

NBD-X

466

535

TET

521

536

Alexa 430

434

541

QSY-7&9
l(MAX)=
560nm

BODIPY R6G-X

529

547

JOE

520

548

Yakima Yellow

531

549

Alexa 532

532

554

VIC

538

554

HEX

535

556

R6G

524

557

BHQ-2
l(MAX)=
579nm
Range=
550-650nm

Alexa 555

555

565

BODIPY 564/570

563

569

BODIPY TMR-X

544

570

Cy3

550

570

Alexa 546

556

573

TAMRA

555

576

Rhodamine Red-X

560

580

BODIPY 581/591

581

591

Redmond Red

579

595

Cy3.5

581

596

 

ROX

575

602

Alexa 568

578

603

Cal Red

583

603

Texas Red

589

610

BODIPY TR-X

588

616

Alexa 594

590

617

BODIPY 630/650-X

625

640

BHQ-3
l(MAX)=
680nm
Range=
620-730nm

LC Red 640

625

640

Alexa 633

632

647

BODIPY 650/665-X

646

660

QSY-21
l(MAX)=
661nm

Alexa 647

650

665

Cy5

649

670

Alexa 660

663

690

BBQ650
l(MAX)=
650nm
Range=
550-750nm

Cy5.5

675

694

Alexa 680

679

702

Alexa 700

702

723

 

Alexa 750

749

775

See dye listings, quencher pair and emission color spectrum

Pdf file contain information on how to choose reporter-quencher pairs for efficient quenching through formation of intramolecular dimers.