ATTO 390, Blue Emission

ATTO® 390 Fluorescent Dye Oligonucleotide Labeling

ATTO® 390 is a premium blue fluorescent dye developed for high-performance fluorescence imaging and analytical applications. The dye exhibits excellent brightness, outstanding photostability, and high fluorescence quantum yield, making it an excellent choice for fluorescence microscopy, molecular diagnostics, and bioanalytical research requiring stable blue fluorescence.

ATTO® 390 is commonly conjugated to DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, peptides, proteins, and other amino-modified biomolecules using NHS ester chemistry, forming a stable amide bond with primary amino groups. Alternative site-specific conjugation methods, including click chemistry, can also be employed depending on the experimental design.

The dye exhibits excitation and emission maxima of approximately 390 nm and 479 nm, respectively, making it compatible with 375 nm, 385 nm, and 405 nm laser systems commonly found on fluorescence microscopes, confocal microscopes, imaging systems, and flow cytometers. Its blue emission provides excellent spectral separation from green, orange, red, and near-infrared fluorophores, making it well suited for multicolor fluorescence experiments.

ATTO® 390-labeled oligonucleotides are widely used for:

• Fluorescence microscopy
• Confocal microscopy
• Fluorescence in situ hybridization (FISH)
• Flow cytometry
• Live-cell imaging
• Molecular diagnostics
• DNA and RNA imaging
• Aptamer imaging
• Cell labeling
• Multiplex fluorescence assays

Bio-Synthesis offers custom ATTO® 390-labeled oligonucleotides for DNA, RNA, PNA, LNA, siRNA, ASOs, molecular beacons, aptamers, and other synthetic nucleic acids. ATTO® 390 can be incorporated at the 5′ end, 3′ end, or internal amino-modified positions, and may be combined with numerous additional modifications including quenchers, biotin, thiol modifiers, amino modifiers, PEG linkers, click chemistry handles, GalNAc, cholesterol, peptides, lipid conjugates, and other custom chemistries.

Each labeled oligonucleotide is synthesized under stringent quality standards and is available with optional HPLC purification, together with analytical characterization by HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence analysis to verify labeling efficiency, sequence identity, and product purity.

 

Trademark Notice: ATTO® is a registered trademark of ATTO-TEC GmbH. Bio-Synthesis provides custom conjugation services using customer-specified or commercially available fluorescent dyes and is not affiliated with or endorsed by ATTO-TEC GmbH.

Product Information

 

Product Name:

ATTO 390, Blue Emission

Category:

Fluorescednt dye label,

Modification Code:

[ATTO390]

Structure:

Purification:

dual HPLC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.

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References/Citations:

1. ATTO-TEC GmbH. ATTO® 390 Fluorescent Label. ATTO-TEC GmbH
2. ATTO-TEC GmbH. ATTO Dyes Product Guide. Fluorescence spectra, photophysical properties, and labeling chemistries.
3. Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155. DOI: 10.1021/cb700248m
4. Hermanson GT. Bioconjugate Techniques. 3rd ed. Academic Press; 2013.

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